1. 제목 : Acid-Responsive Polymeric Nanomaterials for Nucleic Acid Delivery


          and Combined Imaging

2. 연 사 : Young Jik Kwon, Ph.D.

              Departments of Pharmaceutical Sciences, Chemical Engineering and
              Materials Science, and Biomedical Engineering, University of California, Irvine

3. 일 시 : 2011년 4월 4일 오후 5시

4. 장 소 : 301동 1251-1호

5. 내 용 : Nucleic acids (e.g., plasmid DNA, siRNA, etc.) modulate biological/pathological events at a molecular level and nucleic acid-based therapeutics can be logically designed based on target gene sequences. However, cells are not prone to take up and utilize these large and negatively charged macromolecules, which consequently necessitates the use of delivery carriers in order to overcome multiple extracellular and intracellular pathways. This talk will introduce and discuss design, synthesis, characterization, and performance of various novel polymeric nanomaterials that are responsive to an intracellular stimulus (i.e., mildly acidic pH), including 1) polymeric nanoparticles differentially degradable in the endosome, resulting in enhanced transfection via improved endosomal escape and targeted intracellular release/localization of their payloads, 2) highly versatile and efficient polymeric gene carriers that structurally and functionally mimic viral vectors, 3) viral/nonviral hybrid nanoparticles that incorporate the advantages of both viral and nonviral vectors for synergistic gene therapy, and 4) an emerging concept of achieving gene silencing that is triggered only upon generating multiple optical signal changes (i.e., nanotheragnostics). An extended utility of the acid-degradable chemistry in addressing a fundamental limitation in recovering proteins and nucleic acids in their native forms, using degradable polyacrylamide gel electrophoresis (D-PAGE), will also be presented. One of the immediate uses of this novel but simple D-PAGE technique would be investigating interactions between proteins and nucleic acids. This talk will demonstrate crucial roles of interdisciplinary approaches to tackle key biomedical challenges.

6. 약 력 : Assistant Professor, Pharmaceutical Sciences

               Assistant Professor, Chemical Engineering & Materials Science

               Assistant Professor (Adjunct), Biomedical Engineering

 

               Ph.D., University of Southern California, 2003, Chemical Engineering

               M.S., University of Southern California, 2000, Chemical Engineering

               B.S., Inha University, South Korea, 1998, Biological Engineering

 

 

※ 문  의 : 기계항공공학부  전누리 교수 (880-7111)